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[ basic instructions / advanced instructions ]

The 16S rRNA gene can provide a valuable introduction to phylogenetic analysis and bacterial classification, but its use in the classroom can be troublesome for both students and instructors - especially for large classes.

This tool helps instructors by generating unique sequences that can be easily distributed to a classroom (for up to 1000 students). It provides a set of easy-to-follow instructions for students, and an answer key for instructors to help evaluate student performance. The sequences are randomly selected from a set of well-known genera. We "evolve" the sequences by modifying the small number of bases. The changes preserve rRNA secondary structure and avoid highly conserved positions.

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*Class Name (for your reference):

(for example, MMG_331_Spring_08)

*Total # of Students (max. 1000):

*# Sequences per Student (max. 10):

genera set: bacteria and archaea
bacteria
medically-important bacteria

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Questions/comments: rdpstaff@msu.edu

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RDP: Release 11

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