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Assignment Generator :: Advanced Instructions

 

Scientists use different methods to identify bacteria such as microscopy and biochemical tests. A different approach uses the sequencing of the 16S rRNA gene. This gene is highly conserved in bacteria (and other organisms) and strongly correlated with their evolutionary history. Therefore, sequencing this gene provides a good idea of the identity of the bacteria without growing and isolating them. This methodology is highly valued and widely used in many scientific fields as diverse as medicine, plant pathology and geochemistry, among others.

1. Download unknown sequences

This file is in fasta format that can be opened with text editors such as TextEdit on Mac OS X and Wordpad on PC. Do not use Notepad on PC to view the file as it will show the string of text on a single line. The format should appear as follows. The first line is devoted to the name of the sequence and always begins with a right caret followed by the name and a carriage return. The sequence begins on the second line and the sequence text soft wraps (no carriage return between lines) until the second and last carriage return of the file signifies the end of the sequence.

>ID "Student name" (carriage return)
SEQUENCE HERE…END (carriage return)

2. Classify sequences into the taxonomy